Part:BBa_K2588017

Alcohol dehydrogenase 2 from Saccharomyces cerevisiae

We designed ADH2 as a part of the lure module to make E. coli produce 3-methyl-1-butanol to attract mosquitoes. ADH2 as well as kivD is needed to convert 2-ketoisocaproate into 3-methyl-1-butanol.¹

Usage and Biology

ADH2 is the glucose-repressible alcohol dehydrogenase 2. It naturally occurs in Saccharomyces cerevisiae. It is NAD-dependent and normally converts ethanol into acetylaldehyde. But it can act on a variety of primary unbranched aliphatic alcohol such as 2-ketoisocaproate. We expressed ADH2 in E. coli to use it as part of our Lure Module. Together with kivD it converts 2-ketoisocaproate, which was prepared by LeuABCD, into 3-methyl-1-butanol.²

ADH2 is part of the 3MB module, BBa_K2588024.

References

1. Yeastgenome.org. (2018). ADH2 | SGD. [online] Available at: https://www.yeastgenome.org/locus/S000004918 [Accessed 18 Oct. 2018
2. Connor, M. R., Cann, A. F. & Liao, J. C. 3-Methyl-1-butanol production in Escherichia coli: random mutagenesis and two-phase fermentation. Appl. Microbiol. Biotechnol. 86, 1155–1164 (2010).

Sequence and Features




Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 51
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 809
  
• 1000
  COMPATIBLE WITH RFC[1000]